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发表于 2016-11-25 15:55:29
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PMID- 27783675为例，现在都提供全名了
PMID- 27783675
OWN - NLM
STAT- In-Data-Review
DA - 20161026
LR - 20161107
IS - 1553-7374 (Electronic)
IS - 1553-7366 (Linking)
VI - 12
IP - 10
DP - 2016 Oct
TI - DNA Polymerase kappa Is a Key Cellular Factor for the Formation of Covalently
Closed Circular DNA of Hepatitis B Virus.
PG - e1005893
LID - 10.1371/journal.ppat.1005893 [doi]
AB - Hepatitis B virus (HBV) infection of hepatocytes begins by binding to its
cellular receptor sodium taurocholate cotransporting polypeptide (NTCP), followed
by the internalization of viral nucleocapsid into the cytoplasm. The viral
relaxed circular (rc) DNA genome in nucleocapsid is transported into the nucleus
and converted into covalently closed circular (ccc) DNA to serve as a viral
persistence reservoir that is refractory to current antiviral therapies. Host DNA
repair enzymes have been speculated to catalyze the conversion of rcDNA to
cccDNA, however, the DNA polymerase(s) that fills the gap in the plus strand of
rcDNA remains to be determined. Here we conducted targeted genetic screening in
combination with chemical inhibition to identify the cellular DNA polymerase(s)
responsible for cccDNA formation, and exploited recombinant HBV with capsid
coding deficiency which infects HepG2-NTCP cells with similar efficiency of
wild-type HBV to assure cccDNA synthesis is exclusively from de novo HBV
infection. We found that DNA polymerase kappa (POLK), a Y-family DNA polymerase
with maximum activity in non-dividing cells, substantially contributes to cccDNA
formation during de novo HBV infection. Depleting gene expression of POLK in
HepG2-NTCP cells by either siRNA knockdown or CRISPR/Cas9 knockout inhibited the
conversion of rcDNA into cccDNA, while the diminished cccDNA formation in, and
hence the viral infection of, the knockout cells could be effectively rescued by
ectopic expression of POLK. These studies revealed that POLK is a crucial host
factor required for cccDNA formation during a de novo HBV infection and suggest
that POLK may be a potential target for developing antivirals against HBV.
FAU - Qi, Yonghe
AU - Qi Y
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Gao, Zhenchao
AU - Gao Z
AD - National Institute of Biological Sciences, Beijing, China.
AD - Graduate program in School of Life Sciences, Peking University, Beijing, China.
FAU - Xu, Guangwei
AU - Xu G
AUID- ORCID: http://orcid.org/0000-0003-3350-5147
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Peng, Bo
AU - Peng B
AD - National Institute of Biological Sciences, Beijing, China.
AD - Graduate program in School of Life Sciences, Peking University, Beijing, China.
FAU - Liu, Chenxuan
AU - Liu C
AUID- ORCID: http://orcid.org/0000-0002-9960-1302
AD - National Institute of Biological Sciences, Beijing, China.
AD - College of Life Sciences Beijing Normal University, Beijing, China.
FAU - Yan, Huan
AU - Yan H
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Yao, Qiyan
AU - Yao Q
AUID- ORCID: http://orcid.org/0000-0001-7253-0420
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Sun, Guoliang
AU - Sun G
AUID- ORCID: http://orcid.org/0000-0001-5403-5741
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Liu, Yang
AU - Liu Y
AUID- ORCID: http://orcid.org/0000-0002-4952-7714
AD - National Institute of Biological Sciences, Beijing, China.
AD - School of Life Science, Tsinghua University, Beijing, China.
FAU - Tang, Dingbin
AU - Tang D
AD - National Institute of Biological Sciences, Beijing, China.
AD - Graduate program in School of Life Sciences, Peking University, Beijing, China.
FAU - Song, Zilin
AU - Song Z
AD - National Institute of Biological Sciences, Beijing, China.
FAU - He, Wenhui
AU - He W
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Sun, Yinyan
AU - Sun Y
AD - National Institute of Biological Sciences, Beijing, China.
FAU - Guo, Ju-Tao
AU - Guo JT
AD - Baruch S. Blumberg Institute, Doylestown, Pennsylvania, United States of America.
FAU - Li, Wenhui
AU - Li W
AD - National Institute of Biological Sciences, Beijing, China.
LA - ENG
PT - Journal Article
DEP - 20161026
PL - United States
TA - PLoS Pathog
JT - PLoS pathogens
JID - 101238921
PMC - PMC5081172
EDAT- 2016/10/27 06:00
MHDA- 2016/10/27 06:00
CRDT- 2016/10/27 06:00
PHST- 2016/04/23 [received]
PHST- 2016/08/24 [accepted]
AID - 10.1371/journal.ppat.1005893 [doi]
AID - PPATHOGENS-D-16-00905 [pii]
PST - epublish
SO - PLoS Pathog. 2016 Oct 26;12(10):e1005893. doi: 10.1371/journal.ppat.1005893.
eCollection 2016 Oct.
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发表于 2016-11-19 05:39:35
发表于 2016-11-25 10:25:06
